Mitotimer ミトコンドリア
WebMitoTimer transgenic fly line, the coding region of MitoTimer wassubclonedintopUASvector.AUAS-MitoTimertransgenic fly line was established commercially (Genetic Services, Inc.). UAS-MitoTimer flies were crossed with Mef2-Gal4 to obtain Mef2-Gal4UAS-MitoTimer flies.Adultfemaleflieswerecol-lected under light CO 2 … Web(A) heK 293 cells were transfected with pTRe-MitoTimer (MT), exposed to Dox (+) or vehicle (−) for 24 h, then subjected to subcellular fractionation to obtain mitochondria (lanes 1-3) and ...
Mitotimer ミトコンドリア
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WebJan 17, 2024 · A novel MitoTimer reporter gene for mitochondrial content, structure, stress, and damage in vivo. J Biol Chem 2014; 289: 12005–15. Crossref. PubMed. ISI. Google Scholar. 33. Stotland A, Gottlieb RA. alpha-MHC MitoTimer mouse: in vivo mitochondrial turnover model reveals remarkable mitochondrial heterogeneity in the heart. WebMitoTimer is a mutant of the red fluorescent protein DsRed, in which the fluorescence shifts irreversibly from green to red as the protein is oxidized. They targeted the probe to the mitochondrial matrix with a mitochondrial-targeting sequence. MitoTimer can continuously monitor mitochondrial dynamics within cells and is of particular value ...
WebDec 6, 2014 · MitoTimer also provides information about the level of mitochondrial protein import across a population of cells and organelles. High membrane potential is required … WebMitoTimer as a tool for monitoring mitochondrial turnover in vitro and in vivo. 3. Results and discussion 3.1. Constitutive expression of MitoTimer in vitro High membrane potential is required for mitochondrial protein import; thus hypo-polarized mitochondria cannot import newly syn-thesized (green) MitoTimer protein and consequently exhibit a high
WebJul 18, 2024 · Roberta Gottlieb’s lab created another mitochondrion targeted fluorescent protein several years ago, called MitoTimer. First developed in 2000, Timer is a fluorescent protein with two point mutations in DsRed that, in the presence of oxygen, changes from green to red. “We modified Timer to incorporate a mitochondrial targeting sequence from ... WebApr 25, 2014 · Mitochondrial dysfunction plays important roles in many diseases, but there is no satisfactory method to assess mitochondrial health in vivo. Here, we engineered a …
WebJan 1, 2024 · When CAG-CAT-MitoTimer mice were crossbred adiponectin-rtTA-TRE-Cre mice, the double transgenic offspring showed MitoTimer expression revealing mitochondrial network in white adipose tissue following 2 weeks of feeding with doxycline-chow (Fig. 2 e). Similarly, when crossbred with PepCK-Cre mice, the double transgenic offspring showed …
WebPlasmid pTRE-Tight-MitoTimer from Dr. Roberta Gottlieb's lab contains the insert MitoTimer and is published in Autophagy. 2013 Nov 1;9(11):1852-61. doi: … phone repair shop truroWebOct 11, 2013 · MitoTimer is a novel fluorescent reporter protein that can reveal new insights into mitochondrial dynamics within cells. Coupled with organelle flow cytometry, it offers … phone repair shop tampinesWebMitoTimer expression was detected as early as 4 h and persisted (in the presence of continuous Dox) for at least 72 h. To overcome the asynchronous incorporation of continu-ously synthesized new MitoTimer, it was necessary to induce MitoTimer expression with shorter Dox exposure times. Tet-On HEK 293 cells were transfected with 500 ng of pTRE ... phone repair shop tesco kingston parkWebOct 11, 2013 · Flow cytometry analysis of MitoTimer in cells. ( A) Transfected cells were exposed to Dox for 1 h, then cultured for the indicated times (4–144 h), harvested, and … phone repair shop uckfieldWebOct 11, 2013 · MitoTimer was detected in the mitochondrial fraction but absent in the cytosolic fraction, demonstrating specificity of mitochondrial targeting. In the absence of … how do you score the aflsWebMitochondrial dysfunction plays important roles in many diseases, but there is no satisfactory method to assess mitochondrial health in vivo.Here, we engineered a MitoTimer reporter … phone repair shop thornton heathWebOct 21, 2015 · Furthermore, individual mitochondria within cardiomyocytes display a higher red-to-green fluorescence, implying a block in import of newly synthesized MitoTimer that would be caused by the lack of a high membrane potential, indicative of … how do you score points in volleyball