Pherd20t
Web5. sep 2024 · A recombination cassette consisting of genes 34 to 38 of phage JBD30 (anti-CRISPR locus with large flanking regions) in plasmid pHERD20T was previously generated … Web20. okt 2024 · Mutations were confirmed by sequencing. pHERD20T-fleN WT, pHERD20T-vector, pHERD20T-fleN L263W, and pHERD20T-fleN TP255 were electroporated in the …
Pherd20t
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Web(A) AlgUA61V retained the function of inducing alginate production. The wild type algU gene of PAO1 and its variant of CF149 were cloned into pHERD20T, and conjugated into … Web19. júl 2024 · pherd20t-dcas9-grna均进行测序验证。 实施例3利用实施例2构建铜绿假单胞菌的其它必需基因的crispri系统和文库，通过该系统或文库对铜绿假单胞菌pao1的必需基因 …
Web1. okt 2024 · PAO1 harbouring pHERD20T‐dCas9 or pHERD20T‐dCas9‐prtR were used in the tests. (d) Plating efficiencies of 5 × 10 8 /ml bacterial cells (PAO1 harbouring … A previously described31 environmental strain of P. aeruginosa was used as a template to amplify the four cas genes of the Type I-C CRISPR–Cas system genes (cas3, cas5, cas7 and cas8). The genes were cloned into … Zobraziť viac Transformations of P. aeruginosa, E. coli, P. syringae and K. pneumoniae strains were conducted using standard electroporation … Zobraziť viac PAO1IC was grown overnight at 37 °C in LB media. Then 150 μl of overnight culture was added to 4 ml of 0.7% LB top agar and spread on 1.5% LB agar plates supplemented … Zobraziť viac The isogenic DMS3macrIIA4 and acrIC1 phages were constructed using previously described methods60. A recombination cassette, pJZ01, was constructed with homology to the … Zobraziť viac
WebPseudomonas aeruginosa is a widely distributed non-fermentative Gram-negative opportunistic pathogen that is often responsible for nosocomial infections. Gene … WebThe sspA and sspB genes from P . aeruginosa were cloned behind the P BAD promoter in the pHERD20T vector, and conjugated into CF149. (A) Western blot analysis of SspA and …
Web3. mar 2016 · Cyclic diguanosine monophosphate (c-di-GMP) is one of the most important bacterial second messengers that controls many bacterial cellular functions including …
WebWe'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2024 ... bioview fishWeb19. apr 2024 · (A): Examples of microcolonies (enclosed by dash lines) formed by PAO1/pHERD20T, and ΔpslG/pHERD20T, ΔpslG/pG and ΔpslG/pGDM; P BAD-psl and P … dale earnhardt wrangler hatWebThe key point is that the dcas9 gene is located downstream of the P BAD promoter. pHERD20T is an arabinose-inducible vector based on pUCP20T that supports replication … bioview monthlyWebPhage lysates were serially diluted, and 10 μl samples were spotted on lawns of strain ΔPf4 carrying pHERD20T or pHERD20T-pf4r. Discussion In this study, we provided evidences … bioview scannerWeb16. dec 2012 · The pHERD20T vector was digested with EcoRI and HindIII and then infused using the IN-Fusion EcoDry cloning kit (Clontech) following the manufacturer’s instructions. bioview ctcWeb22. aug 2013 · Strains containing pHERD20T-algU WT (PAO1), pHERD20T-algU A61V (CF149), and pHERD20T were streaked on PIA plates supplemented with 300 µg/ml of … dalee electronics crossing flasherbiovineshoot